Researchers from the Bodenmiller Lab at DQBM, with partners at ETH Zurich and University Hospital Basel, present high resolution IMC using oversampling and PSF based deconvolution to reach below 350 nm. The method reveals nuclear foci, mitochondrial networks and fine membranes that classical IMC cannot resolve, and improves segmentation in densely packed tissues.
They show applications from chemotherapy treated ovarian cancer cells to in situ tumor tissues, linking subcellular organization to cell states. The advance keeps IMC’s high multiplexity while pushing into subcellular analysis, opening new routes for spatial proteomics in health and disease.