18.02.2022The Polymenidou group publishes a protocol for the identification of RNA–RBP interactions in subcellular compartments by CLIP-Seq

Cross-linking immunoprecipitation and high-throughput sequencing (CLIP-seq) allows the identification of RNA bound by RNA-binding proteins (RBPs) in vivo and ex vivo with high specificity. In this protocol paper, the Polymenidou lab describe the adaptation of CLIP-seq to identify the specific RNA targets of an RBP (FUS) at neuronal synapses, including subcompartment isolation, RBP–RNA complex enrichment, and upscaling steps.

See Sahadevan, Pérez-Berlanga & Polymenidou, Methods Mol. Biol.