02.01.2024The Krauthammer group develops Fragmentstein: A Novel Tool for cfDNA Fragment Analysis

See Balázs et al., Bioinformatics 2024

The Krauthammer group introduces Fragmentstein, a command-line tool designed to convert non-sensitive cfDNA fragment data into BAM files suitable for standard bioinformatics analyses. This innovative approach facilitates data sharing without compromising sensitive genomic information, enabling analyses like CNV, nucleosome occupancy, and fragment length studies while ensuring data privacy.

Overview of the application test case. Alignment files and publicly accessible fragment coordinate data of the same samples were downloaded. Fragmentstein creates alignment files for each sample using only non-sensitive information. The original alignment files and the alignment files generated by Fragmentstein were subjected to fragment length, copy number, and nucleosome occupancy analysis.